A putative processing enzyme for proenkephalin in bovine adrenal chromaffin granule membranes
نویسندگان
چکیده
منابع مشابه
Interaction of calmodulin with adrenal chromaffin granule membranes.
We have reported that calmodulin bound specifically to catecholamine-containing secretory granules of adrenal medullary chromaftin cells [I]. Binding was dependent upon pM levels of Ca2+ and saturated at a low level (3.3. pmol calmodulin/mg granule membrane protein). Additionally, we observed that the phosphorylation of membrane polypeptides of M, 59 000, 58 000 and 53 000 was stimulated by cal...
متن کاملActive and passive transport of dopamine in chromaffin granule ghosts isolated from bovine adrenal medulla.
A high performance liquid chromatographic (HPLC) procedure is described for the determination of picomole amounts of catecholamines based on the native fluorescence of the compounds. Using this method, it is shown that the ATP-driven uptake of dopamine in resealed chromaffin granule ghosts from bovine adrenal medulla is kinetically linked to the inward translocation of protons (H+) induced by A...
متن کاملAn investigation of the molecular properties and stability of intermediates of proenkephalin in isolated bovine adrenal medullary chromaffin granules.
An antiserum to a synthetic peptide corresponding to residues 95-117 of bovine proenkephalin recognizes all the major intermediates of this prohormone in bovine adrenal medulla (Birch, N. P. and Christie, D. L. (1986) J. Biol. Chem. 261, 12213-12221). This antiserum enabled an investigation of the stability and molecular properties of intermediates in the processing of proenkephalin in bovine a...
متن کاملBovine Chromaffin Granule Membranes Undergo Ca"-regulated Exocytosis in Frog Oocytes
We have devised a new method that permits the investigation of exogenous secretory vesicle function using frog oocytes and bovine chrornaffin granules, the secretory vesicles from adrenal chromaffin cells. Highly purified chrornaffin granule membranes were injected into Xenopus laevis oocytes . Exocytosis was detected by the appearance of dopamineß-hydroxylase of the chrornaffin granule membran...
متن کاملBovine chromaffin granule membranes undergo Ca(2+)-regulated exocytosis in frog oocytes
We have devised a new method that permits the investigation of exogenous secretory vesicle function using frog oocytes and bovine chromaffin granules, the secretory vesicles from adrenal chromaffin cells. Highly purified chromaffin granule membranes were injected into Xenopus laevis oocytes. Exocytosis was detected by the appearance of dopamine-beta-hydroxylase of the chromaffin granule membran...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)84873-1